The aim of this study was to establish the most common causative agents of otomycosis in Duhok
city, and study their sensitivity to the antifungal medications that are commonly prescribed. From
June to august 2021, a total of 90 patients (46 females and 44 males) were clinically examined for
mycotic otitis who attended the outpatient clinic of ear, nose and throat (ENT) department at Azadi
teaching hospital. Ear debris collected by sterile swabs and transferred to the laboratory for direct
microscopic and macroscopic examination by culturing on sabouraud dextrose agar, potato
dextrose agar and CHROM Agar. Antifungal drugs were dissolved with (dimethyl sulfoxide)
DMSO and used for evaluation of antifungal sensitivity by agar well diffusion method against
commonly used antifungal drugs namely; Fluconazole, Itraconazole, Terbinafine, Nysatin,
Amphotericin B, and Clotrimazole. In this study positive fungal infection were found in 88
(97.8%) of the collected samples, and it was more common among patients that aged 40 to 49
years old. Mycological examination revealed the isolation of 24 species belong to 6 genera with
one variety. The most common fungal isolates were Candida (88.9%), followed by Aspergillus
(28.8%) and non-identified yeast (25%), then Penicillium (8.9%). Candida krusei (44.4%) and
candida albicans (16.7%) followed by Aspergillus niger (15.6%) were the predominant species
isolates. In vitro antifungal susceptibility pattern analysis revealed that yeast had the highest
sensitivity to (itraconazole) followed by (amphotericin B). while moulds isolates, their highest
sensitivity was to (clotrimazole) followed by (terbinafine).